experimental models h9c2 atcc crl 1446 tm hek293t atcc crl 3216 tm cos 7 atcc crl 1651 tm  (ATCC)

Journal: Light, Science & Applications

Article Title: Interferometric Image Scanning Microscopy for label-free imaging at 120 nm lateral resolution inside live cells

doi: 10.1038/s41377-026-02210-y

Figure Lengend Snippet: a Overview of a COS-7 cell. Scale bar 10 μ m. Arrows mark individual organelles that are visible in this particular optical section. Nucleus (N), mitochondria (M), actin cytoskeleton (A), plasma membrane and lamellipodum (L), the endoplasmic reticulum (ER) as well as vesicles (V). Notably, positive and negative contrast of the same type of organelle indicate slightly different axial positions with respect to the optical section. b Upper close-up from a showing ER, vesicles and lamellipodium. Scale bar 2 μ m. c Lower close-up from a showing ER and lamellipodium. Scale bar 2 μ m. d Confocal iSCAT evaluated for the close-up of g as indicated with white dashed square. (Left) Closed pinhole and (right) open pinhole analysis. e iISM-APR of the same region as d . Scale bars d , e 200 nm. f Line profiles of interference contrast of vesicle cross-section as indicated in d , e for closed pinhole (green), open pinhole (blue) and iISM-APR (red). g iISM-APR timelapse of close-up in c after additional background correction to increase visibility of organelles on flat-fielded background (see Methods). h Timelapse of close-up in g with exemplary four consecutive time points ( t 0 − t 4 ) at time increments of about 8.2 s. Upper red arrows indicate vesicle motion, lower red arrows indicate ER remodeling. Scale bars g , h 1 μ m

Article Snippet: African green monkey kidney COS-7 cells (ATCC CCL-70) were cultivated in DMEM supplemented with 10% heat-inactivated FBS.

Techniques: Clinical Proteomics, Membrane